https://www.chronislab.com/our-vision daily 0.75 2024-10-22 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/9185f6f6-571f-42ab-972d-611565c17eb7/JCP2011.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/ca796db4-8f9c-4885-a55e-d1bd45b731b3/GenomeResearch2009.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/393808db-feaa-4e5a-ae94-1e6adc3d082f/nature2020_apj.png Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/e778efd2-e5a0-45ce-8af5-69eb30264bd5/CellStemCell_chronis.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/a687d385-5ddb-4161-b792-badbe1f19cd1/nature+cell+biologyRS.png Publications - 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Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/da41be97-8423-409b-8e1a-9c78b2902a40/NAR2012.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/f22813ce-01dc-4e7b-992e-1c7c5f4d884c/msb.v10.12.cover.jpg Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/22e27239-a9b1-4723-96cf-48c420a649d9/StemCellReports_Chronis.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/7e490ce7-eacc-4d76-9305-3176322cfd9a/CellStemCell_denholtz.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/ec86dd77-a0a8-489a-8493-1a900af19c02/MolCell_Xu.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/8d4f5bfc-453a-4119-9f49-54d2d4cb804f/CellReports_Patel.gif Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.chronislab.com/new-page daily 0.75 2024-08-23 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/1636146324256-CUR3C6VHTGU8EJLIT07J/IMG_3478+2.JPG Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/1636148208171-69T0T0ZF7DEDK346GT9T/Attach4586_20180514_220147.jpg Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/1636387860339-3GVCELNBW87HKNLJ1MZI/Image+from+iOS.jpg Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/1659455876016-LL03V0BMTJP994HWBW8Y/EVA.jpeg Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/922c30cc-a98b-4eb1-8916-45dccc5a7d64/MD_PHD+Headshot.jpeg Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/b9d245b8-848e-4b0f-a53a-1c6cb9c8086b/menhart_profilepic.jpg Team https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/631c0154-ec32-4900-959b-8f221fce8cab/AliF_edited.jpg Team https://www.chronislab.com/new-page-1 daily 0.75 2021-11-16 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/e2f9f267-4e2b-4353-8979-72871cff4723/Cell_Atlas.jpg Research - A Multi-Scale Analysis of Cell fate Commitment and Reprogramming How are pluripotent Stem Cells “programmed” to generate all bodily cell types during development, and how can somatic cells be “reprogrammed” to an ESC-like state? To delineate the events that drive cell fate decisions we use genomic and single cell approaches to identify markers and cell type specific signatures that can distinguish cell states and the regulatory mechanisms that govern the production of such diversity. By applying innovative technologies, we create detailed atlases of regulatory DNA and characterize novel chromatin states and their function in the establishment and maintenance of cell identity. We aim to understand the impact of chromatin reprogramming on cell fate commitment in normal development and during initiation and cancer progression. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/b484c893-e0f0-4828-bb20-d268a9a6cfde/website3.jpg Research - Mechanism of Transcription factor assembly and binding dynamics in gene regulation Activation and repression of developmental gene expression programs are controlled by the combinatorial actions of sequence specific transcription factors (TFs) and their effects on 3D genome organization. Yet, how TFs identify and select their genomic targets from thousands of potential binding sites remains unclear. We are assessing the contribution of DNA sequence variation, chromatin content, RNA presence and modalities of TF interactions on genomic target selection. Current research in the lab is aimed at understanding how reprogramming transcription factors facilitate the loss or destabilization of somatic cell identities by repressing diverse somatic gene regulatory programs and how these pathways potentially contribute to malignancies that involve de-differentiation of somatic tissues. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/7f08e441-6942-425e-8038-19759a1db6d8/TOOL_DEV.jpg Research - Novel tools to study Epigenetic regulation, Chromatin architecture, and TF dynamics While traditional transcriptomic, epigenetic, and proteomic profiling methods have provided key insights in gene regulation, chromatin structure and protein function they typically rely on large populations of cells, are largely correlative, and fail to capture dynamic transitions in a direct manner. We are developing novel co-assays that measure protein binding and transcriptional output within single cells of heterogeneous populations to measure in a quantitative manner the causal relationships between transcription factor binding and gene regulation. https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/2c87c1c2-93ff-4330-9b8c-e20b5aa3d1b3/Celulas+estaminais+mesenquimais+em+cultura.jpg Research - Engineering customized Hematopoietic Stem, Progenitor and Mature Blood cells Hematopoietic Stem Cells (HSCs) represent a rare cell type capable of reconstituting all blood lineages, and through transplantation the only curative therapy for the treatment of blood disorders. In vitro generation of clinically relevant blood progenitors holds great promise for regenerative medicine and makes disease modeling, toxicity screening, and immunotherapy tangible. Significant challenges in HSC expansion and failure to differentiate HSCs from pluripotent stem cells in vitro, makes direct conversion of somatic cells to HSCs (iHSCs) and/or their differentiated descendants into an attractive approach. We use novel computational and systems biology approaches to define and manipulate key cis regulatory elements that contribute to HSC specification and the factors that contribute to differentiation towards lymphoid lineages. https://www.chronislab.com/new-page-2 daily 0.75 2021-11-09 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/1636066619673-B7ORTJVEJRC2XCW9A7HQ/unsplash-image-idhx-MOCDSk.jpg Careers - Interested in joining the lab? We are always interested in motivated individuals with an interest in Pluripotent and Blood Stem Cells, Genomics, Chromatin, Aging and/or technology development. If you are interested in translating stem cell discoveries into patient-specific therapies we want to hear from you! For prospective GEMS PhD students we are part of the Cell Biology and Regenerative Medicine, Cancer Biology, and Molecular and Structural Biology research concentrations. For prospective bioengineering students we are a member of the bioinformatics and quantitative biology research center (CBQB). Interested students should email chronis@uic.edu to discuss potential rotation projects. https://www.chronislab.com/home daily 1.0 2024-10-22 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/66abb96e-6735-4ca7-91fe-54be33560edc/Primed_%0ENaive_iPSCs.jpg Home Capturing the Naive (red) and Primed (green) states of hESCs https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/41cb91bf-8093-4882-a2f2-39e6a39b644c/FISH_gene+expression.jpg Home Gene expression (red) in the nucleus of Naive iPSCs https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/43449416-8cfc-448c-b882-803e0fdaecbb/20211025+p0_0002_zoverlay.jpg Home ex vivo expansion of Hematopoietic Stem Cells https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/05905632-0069-48ea-a07d-fc030d7315f7/Screen+Shot+2021-11-02+at+7.40.18+AM.jpg Home - University of Illinois at Chicago We are located in a vibrant area of Chicago, only minutes away from downtown. Our laboratory is on the second floor of the Molecular Biology Research building (MBRB) and we are part of the multi-disciplinary department of Biochemistry and Molecular Genetics within the college of Medicine. We are also affiliated with the Center for Bioinformatics and Quantitative Biology and the UIC Cancer Center. The research focus areas within the research center are stem cells and developmental biology, genomics, structural biology, cell signaling and diverse aspects of cancer biology. Our address: Department of Biochemistry and Molecular Genetics University of Illinois at Chicago, MBRB 2370 900 S. Ashland Avenue, Chicago, IL 60607-7170 Office no 312-355-8012 Lab no 312-355-8011 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/57c3cacb-5f6a-4ebb-94b6-07666206fcf5/Impetus+logo.png https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/b12f0a78-3c9f-45d8-bcff-e29f10a22218/Screenshot+2023-09-18+at+10.35.17+AM.png https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/793853cf-1902-4e28-93a2-3fff4fff465c/Screenshot+2023-09-18+at+10.30.41+AM.png https://www.chronislab.com/lab-resources daily 0.75 2021-11-16 https://images.squarespace-cdn.com/content/v1/5ec321c2af33de48734cc929/1618497259178-6XJGK9GR6YAVBQL5L519/20140301_Trade-151_012-2.jpg Lab Resources https://images.squarespace-cdn.com/content/v1/5ec321c2af33de48734cc929/1607694583486-2PQT0LQ193RL7MCB6DX4/20140228_Trade+151_0046.jpg Lab Resources https://images.squarespace-cdn.com/content/v1/5ec321c2af33de48734cc929/1607694644871-IC85FNH781UNZSZEGHDR/Aro+Ha_0428.jpg Lab Resources https://www.chronislab.com/ourvision daily 0.75 2021-11-16 https://images.squarespace-cdn.com/content/v1/6180fb67dfa7a7572c6b98b8/d4a40065-2117-4654-8387-4a47f7d51ae4/Screen+Shot+2021-11-02+at+10.02.27+PM.jpg Our Vision - Our Vision Cell fate specification is among the most fundamental processes in biology and is exerted physiologically during stem cell differentiation, pathologically during cancer stem cell formation, and can be engineered with reprogramming approaches. All these processes involve activation of target programs and inhibition of donor cell programs and are controlled by sequence-specific DNA binding transcription factors (TFs) and their diverse interactions with the chromatin landscape. We aim to understand how TFs decode mammalian genomes to generate the diverse cell identities that arise during development, cellular reprogramming and pathological conditions. To this end we employ multi-omic approaches, genome engineering, single cell tool development and bioinformatic analysis to delineate the TF-driven mechanisms that determine cell fate specification. Our ultimate goal is to engineer customized stem cells and their differentiated progeny for clinical applications while exploring the mechanisms that govern organ aging and regeneration and tumor growth.